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Cell Seeding Density Calculator
Calculate cells per well and pipette volume for all six plate formats from one hemocytometer count and confluency target.
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Cell Seeding Density Calculator
HEK293T · 30% seeding
Selected: HEK293T
Time estimate assumes exponential growth; contact inhibition near confluence may slow growth above ~70%
~38.2 h to reach 80% confluency
6W✓
Cells/well
655,500
Volume to add
345 µL
12W✓
Cells/well
262,200
Volume to add
138 µL
24W✓
Cells/well
131,100
Volume to add
69 µL
48W✓
Cells/well
65,550
Volume to add
34.5 µL
96W✓
Cells/well
22,080
Volume to add
11.62 µL
384W✓
Cells/well
6,900
Volume to add
3.63 µL
Cell Seeding Protocol
Generated: 6/9/2026, 5:04:32 AM
Inputs
- Cell line: HEK293T
- Cells/cm² at confluency: 230,000
- Stock concentration: 2,000,000 cells/mL
- Viability: 95%
- Seeding confluency: 30%
- Assay confluency target: 80%
- Doubling time: 27 h
Plate grid
| Format | Cells/well | Volume |
|---|---|---|
| 6W | 655,500 | 345 µL |
| 12W | 262,200 | 138 µL |
| 24W | 131,100 | 69 µL |
| 48W | 65,550 | 34.5 µL |
| 96W | 22,080 | 11.62 µL |
| 384W | 6,900 | 3.63 µL |
Time to 80% confluency: ~38.2 h
Warnings
- Time estimate assumes exponential growth; contact inhibition near confluence may slow growth above ~70%