Pepkio · Tool workspace

Tm / Annealing Temperature Calculator

Calculate primer Tm and suggested annealing temperature for single pairs or batch CSV, with QC flags and exportable results.

Primer name (optional)

Forward sequence

Reverse sequence

Polymerase

DMSO % — 0

Betaine (M)

Empirical Tm correction (−0.75°C per 1 M); optimize annealing empirically in the lab.

Results

Enter sequences and click Calculate to see Tm, Ta, and QC flags.