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pH Buffer Recipe Calculator

Get weighed buffer recipes with temperature-corrected pH, ionic strength, stock dilution, and copy-ready protocols.

Ionic strength20 mM

Results

Acid form
6.178 g Tris hydrochloride
Base form
1.308 g Tris base
Final volume
1000.0 mL
Ionic strength
20 mM

gravimetric preparation

  1. Weigh 6.178 g Tris hydrochloride and 1.308 g Tris base.
  2. Dissolve in ~80% of final volume (1000 mL) using 25°C water.
  3. Adjust to final volume 1000.0 mL; verify pH at 25°C (target pH 7.5).

titration preparation

  1. Weigh 6.178 g Tris hydrochloride (acid form).
  2. Dissolve in ~80% of final volume; titrate with NaOH or HCl to pH 7.5 at 25°C.
  3. Bring to 1000.0 mL final volume.
  4. Avoid overshooting pH — correcting back changes ionic strength and capacity.

Compatibility

  • Tris reacts with aldehydes and some amine-reactive crosslinkers; not ideal for protein carbonylation assays.