Pepkio Tools

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Tools

  • GEO metadata parser

    Extracts structured, pipeline-friendly metadata from GEO accessions (e.g. GSE series, GSM samples) and returns a normalized summary.

  • CRISPR guide checker

    Lightweight heuristic checks for CRISPR guide RNAs: basic sequence validation, GC content, homopolymer flags, and optional PAM pattern matching.

  • Bulk RNA-seq normalization

    Normalizes a bulk RNA-seq count matrix using CPM and log2(CPM + 1). Intended as a compute-friendly building block for downstream analyses.

  • Pathway overlap visualizer

    Computes overlap between a user gene set and pathway gene sets using simple set metrics (intersection size and Jaccard index).

  • Single-cell marker finder

    Suggests likely cell types from a list of top expressed genes using a small curated marker dictionary and simple overlap scoring.

  • Gene symbol cleaner

    Normalizes gene symbols to HGNC-approved symbols using MyGene.info. Returns a mapping table for ambiguous or outdated symbols.

  • VCF formatter

    Normalizes VCF text: sorts header lines, orders INFO fields, and validates basic structure.

  • FASTQ QC visualizer

    Analyzes FASTQ quality metrics: per-base quality, GC content, and pass/fail summary.